Etude d'un procédé d'encapsulation de microARN en milieu supercritique (thèse 2017-2020)
Activités
Encapsulation / microARN / CO2 supercritique / SAS
Publications scientifiques au M2P2
2023
Mathieu Martino, Adil Mouahid, Michelle Sergent, Camille Desgrouas, Catherine Badens, et al.. Supercritical millifluidic process for siRNA encapsulation in nanoliposomes for potential Progeria treatment (ex-vivo assays). Journal of Drug Delivery Science and Technology, 2023, 87, pp.104804. ⟨10.1016/j.jddst.2023.104804⟩. ⟨hal-04254108⟩ Plus de détails...
A millifluidic process working in continuous mode for the preparation of nanoliposomes using supercritical CO2 has been developed. Nanoliposomes with an average diameter ranging between 123.9 ± 3.0 and 165.7 ± 1.6 nm depending on the operating conditions were obtained. The effects of pressure (90–150 bar), temperature (35–45 °C) and phospholipid mass ratio (0.1–1.9 wt%) in feed solution on liposome sizes were investigated. The concentration of phospholipids was found to be the most significant parameter for controlling the mean diameter of nanoliposomes while pressure and temperature had a minor influence on liposomes’ properties. The encapsulation of siRNAs targeting the LMNA gene by nanoliposomes obtained with the millifluidic process was achieved at optimized operating conditions (150 bar, 35 °C and a phospholipid mass ratio in the feed solution of 0.1 wt%). The resulting formulations were compared with commercial transfection agents in ex vivo assays. These assays showed a decrease in the expression of the encoded protein lamin A for the formulations obtained with the process developed in this work. Therefore, the use of siRNAs targeting LMNA, encapsulated by nanoliposomes represents a potential new therapeutic approach for the treatment of progeria.
Mathieu Martino, Adil Mouahid, Michelle Sergent, Camille Desgrouas, Catherine Badens, et al.. Supercritical millifluidic process for siRNA encapsulation in nanoliposomes for potential Progeria treatment (ex-vivo assays). Journal of Drug Delivery Science and Technology, 2023, 87, pp.104804. ⟨10.1016/j.jddst.2023.104804⟩. ⟨hal-04254108⟩
Journal: Journal of Drug Delivery Science and Technology
Mathieu Martino, Hugo Taligrot, Clémence Cordier, Philippe Moulin. Supercritical fluid treatment of organic membranes. Journal of Membrane Science, 2022, 661, pp.120892. ⟨10.1016/j.memsci.2022.120892⟩. ⟨hal-04063877⟩ Plus de détails...
Membrane processes are used for drinking water production and medical applications to remove and/or produce viruses using organic membranes. It appears that backwashing is not sufficient to remove all the viruses stopped by the membrane. The use of "one-shot" membranes or chlorinated solution filtration can be considered with an additional cost and accelerated aging of the membranes respectively. The inactivation of bacteria, spores and/or viruses has been demonstrated by the use of supercritical fluid. The use of a supercritical CO 2 treatment of organic membranes is studied with the aim to investigate the impact of this treatment on the membrane performances and on virus destruction. It is demonstrated that whatever the operating conditions, the membrane materials and the application modes, the permeability and the retention of the membrane are not impacted. TEM observation of the viruses after treatment shows no virus integrity.
Mathieu Martino, Hugo Taligrot, Clémence Cordier, Philippe Moulin. Supercritical fluid treatment of organic membranes. Journal of Membrane Science, 2022, 661, pp.120892. ⟨10.1016/j.memsci.2022.120892⟩. ⟨hal-04063877⟩
Mathieu Martino, Hugo Taligrot, Clémence Cordier, Philippe Moulin. Supercritical fluid treatment of organic membranes. Journal of Membrane Science, 2022, 661, pp.120892. ⟨10.1016/j.memsci.2022.120892⟩. ⟨hal-03967095⟩ Plus de détails...
Paolo Trucillo, Mathieu Martino, Ernesto Reverchon. Supercritical Assisted Production of Lutein-Loaded Liposomes and Modelling of Drug Release. Processes, 2021, 9 (7), pp.1162. ⟨10.3390/pr9071162⟩. ⟨hal-03515201⟩ Plus de détails...
In this work, a lipophilic ophthalmic drug, lutein, has been entrapped in liposomes, using a supercritical assisted process. Effects of pressure, temperature, and drug to lipid ratio variation were studied on mean diameters and lutein encapsulation efficiency. Liposomes with diameters between 153 ± 38 and 267 ± 56 nm were produced, and lutein encapsulation efficiencies between 86.5 ± 0.4% and 97.8 ± 1.2% were obtained. A Scanning Electron Microscope confirmed spherical shape and mean dimensions of vesicles. The variation of temperature for the production of liposomes showed a significant impact on lutein retention time in the double lipidic layer. Lutein drug release from liposomes produced at 35 °C ended in almost 4.5 days; whereas, liposomes produced at 40 °C showed a faster lutein release in 3 days; then, vesicles obtained at 45 °C released their lutein content in only 2 days. Drug release raw data were well-fitted using Weibull model (R2 up to 99%).
Paolo Trucillo, Mathieu Martino, Ernesto Reverchon. Supercritical Assisted Production of Lutein-Loaded Liposomes and Modelling of Drug Release. Processes, 2021, 9 (7), pp.1162. ⟨10.3390/pr9071162⟩. ⟨hal-03515201⟩
Mathieu Martino, Adil Mouahid, Paolo Trucillo, Elisabeth Badens. Elaboration of Lutein‐Loaded Nanoliposomes Using Supercritical CO2. European Journal of Lipid Science and Technology, 2021, 123 (4), pp.2000358. ⟨10.1002/ejlt.202000358⟩. ⟨hal-03334357⟩ Plus de détails...
A batch process for producing lutein-loaded liposomes using supercritical CO2 is studied. The effects of the variation of pressure (10 and 15 MPa), temperature (308, 313, and 318 K), and lutein to lipid ratio (0.5 and 1 wt%) on the liposome average size and size distribution are investigated, as well as on the encapsulation efficiency (EE) of lutein. This process is worked in a repeatable manner and is allowed the production of nanoliposomes with mean diameters (MDs) ranging from 65 ± 33 to 77 ± 40 nm, obtaining lutein EEs ranging from 82.1 ± 3.7% to 91.9 ± 2.9%. Temperature, pressure, and lutein to lipid ratio seem to have no impact on size, size distribution, and EE on formed liposomes. The use of low temperatures and low pressures allows the obtainment of liposomes with diameters less than 100 nm and limits the process energy cost. Moreover, the supercritical CO2-assisted batch process effectively encapsulates lutein into liposome, an antioxidant molecule used for the prevention of retinal damage. Liposomes formed by this supercritical process have the desired characteristics for human target delivery. Practical applications: This work on the optimization of a process for developing liposomes in a supercritical environment has applications in medicine. Indeed, the liposomes formed with this process are nanoliposomes with a size of less than 80 nm. In addition, excellent lutein EEs (hydrophobic molecules) show that the liposomes formed constitute excellent coating matrices for the protection of active ingredients. These reasons make these liposome matrices applicable in nanomedicine (injection of sensitive drugs requiring protection before injection). The elaboration process also makes it possible to form liposomes with desired properties by changing pressure, temperature, or lecithin concentration. Therefore, this work focuses on the properties of liposomes as a function of the operating conditions.
Mathieu Martino, Adil Mouahid, Paolo Trucillo, Elisabeth Badens. Elaboration of Lutein‐Loaded Nanoliposomes Using Supercritical CO2. European Journal of Lipid Science and Technology, 2021, 123 (4), pp.2000358. ⟨10.1002/ejlt.202000358⟩. ⟨hal-03334357⟩
Journal: European Journal of Lipid Science and Technology
Mathieu Martino, Adil Mouahid, Paolo Trucillo, Elisabeth Badens. Elaboration of Lutein‐Loaded Nanoliposomes Using Supercritical CO2. European Journal of Lipid Science and Technology, 2021, 123 (4), pp.2000358. ⟨10.1002/ejlt.202000358⟩. ⟨hal-03597665⟩ Plus de détails...
A batch process for producing lutein-loaded liposomes using supercritical CO2 is studied. The effects of the variation of pressure (10 and 15 MPa), temperature (308, 313, and 318 K), and lutein to lipid ratio (0.5 and 1 wt%) on the liposome average size and size distribution are investigated, as well as on the encapsulation efficiency (EE) of lutein. This process is worked in a repeatable manner and is allowed the production of nanoliposomes with mean diameters (MDs) ranging from 65 ± 33 to 77 ± 40 nm, obtaining lutein EEs ranging from 82.1 ± 3.7% to 91.9 ± 2.9%. Temperature, pressure, and lutein to lipid ratio seem to have no impact on size, size distribution, and EE on formed liposomes. The use of low temperatures and low pressures allows the obtainment of liposomes with diameters less than 100 nm and limits the process energy cost. Moreover, the supercritical CO2-assisted batch process effectively encapsulates lutein into liposome, an antioxidant molecule used for the prevention of retinal damage. Liposomes formed by this supercritical process have the desired characteristics for human target delivery. Practical applications: This work on the optimization of a process for developing liposomes in a supercritical environment has applications in medicine. Indeed, the liposomes formed with this process are nanoliposomes with a size of less than 80 nm. In addition, excellent lutein EEs (hydrophobic molecules) show that the liposomes formed constitute excellent coating matrices for the protection of active ingredients. These reasons make these liposome matrices applicable in nanomedicine (injection of sensitive drugs requiring protection before injection). The elaboration process also makes it possible to form liposomes with desired properties by changing pressure, temperature, or lecithin concentration. Therefore, this work focuses on the properties of liposomes as a function of the operating conditions.
Mathieu Martino, Adil Mouahid, Paolo Trucillo, Elisabeth Badens. Elaboration of Lutein‐Loaded Nanoliposomes Using Supercritical CO2. European Journal of Lipid Science and Technology, 2021, 123 (4), pp.2000358. ⟨10.1002/ejlt.202000358⟩. ⟨hal-03597665⟩
Journal: European Journal of Lipid Science and Technology
Paolo Trucillo, Mathieu Martino, Ernesto Reverchon. Supercritical Assisted Production of Lutein-Loaded Liposomes and Modelling of Drug Release. Processes, 2021, 9 (7), pp.1162. ⟨10.3390/pr9071162⟩. ⟨hal-03515201⟩ Plus de détails...
In this work, a lipophilic ophthalmic drug, lutein, has been entrapped in liposomes, using a supercritical assisted process. Effects of pressure, temperature, and drug to lipid ratio variation were studied on mean diameters and lutein encapsulation efficiency. Liposomes with diameters between 153 ± 38 and 267 ± 56 nm were produced, and lutein encapsulation efficiencies between 86.5 ± 0.4% and 97.8 ± 1.2% were obtained. A Scanning Electron Microscope confirmed spherical shape and mean dimensions of vesicles. The variation of temperature for the production of liposomes showed a significant impact on lutein retention time in the double lipidic layer. Lutein drug release from liposomes produced at 35 °C ended in almost 4.5 days; whereas, liposomes produced at 40 °C showed a faster lutein release in 3 days; then, vesicles obtained at 45 °C released their lutein content in only 2 days. Drug release raw data were well-fitted using Weibull model (R2 up to 99%).
Paolo Trucillo, Mathieu Martino, Ernesto Reverchon. Supercritical Assisted Production of Lutein-Loaded Liposomes and Modelling of Drug Release. Processes, 2021, 9 (7), pp.1162. ⟨10.3390/pr9071162⟩. ⟨hal-03515201⟩
Adil Mouahid, Kanitta Seengeon, Mathieu Martino, Christelle Crampon, Avery Kramer, et al.. Selective extraction of neutral lipids and pigments from Nannochloropsis salina and Nannochloropsis maritima using supercritical CO2 extraction: Effects of process parameters and pre-treatment. Journal of Supercritical Fluids, 2020, 165, pp.104934. ⟨10.1016/j.supflu.2020.104934⟩. ⟨hal-02960133⟩ Plus de détails...
Supercritical CO2 extraction experiments were conducted to investigate the effects of pretreatment and process parameters on neutral lipids, chlorophylls and carotenoids recovery on two species of Nannochloropsis. For Nannochloropsis maritima, a factorial experimental design was performed (P: [100-300] bar, T: [313-333] K). The highest extraction yields were obtained at the highest pressures and temperatures. Two drying modes, ring drying and air flow drying, were compared. Although total extraction yield and extraction kinetics were observed to be greater using air flow dried microalgae, extracts from this drying method resulted in partial degradation of glycerides in free fatty acids. Ring dried extracts maintained the same neutral lipid composition as the initial biomass. Based on these results, ring dried Nannochloropsis salina was extracted using supercritical CO2 at 333 K and both 300-400 bar. Extraction curves were modelled using the Sovova's mathematical model.
Adil Mouahid, Kanitta Seengeon, Mathieu Martino, Christelle Crampon, Avery Kramer, et al.. Selective extraction of neutral lipids and pigments from Nannochloropsis salina and Nannochloropsis maritima using supercritical CO2 extraction: Effects of process parameters and pre-treatment. Journal of Supercritical Fluids, 2020, 165, pp.104934. ⟨10.1016/j.supflu.2020.104934⟩. ⟨hal-02960133⟩
Adil Mouahid, Kanitta Seengeon, Mathieu Martino, Christelle Crampon, Avery Kramer, et al.. Selective extraction of neutral lipids and pigments from Nannochloropsis salina and Nannochloropsis maritima using supercritical CO2 extraction: Effects of process parameters and pre-treatment. Journal of Supercritical Fluids, 2020, 165, pp.104934. ⟨10.1016/j.supflu.2020.104934⟩. ⟨hal-03232116⟩ Plus de détails...
Supercritical CO2 extraction experiments were conducted to investigate the effects of pretreatment and process parameters on neutral lipids, chlorophylls and carotenoids recovery on two species of Nannochloropsis. For Nannochloropsis maritima, a factorial experimental design was performed (P: [100-300] bar, T: [313-333] K). The highest extraction yields were obtained at the highest pressures and temperatures. Two drying modes, ring drying and air flow drying, were compared. Although total extraction yield and extraction kinetics were observed to be greater using air flow dried microalgae, extracts from this drying method resulted in partial degradation of glycerides in free fatty acids. Ring dried extracts maintained the same neutral lipid composition as the initial biomass. Based on these results, ring dried Nannochloropsis salina was extracted using supercritical CO2 at 333 K and both 300-400 bar. Extraction curves were modelled using the Sovova's mathematical model.
Adil Mouahid, Kanitta Seengeon, Mathieu Martino, Christelle Crampon, Avery Kramer, et al.. Selective extraction of neutral lipids and pigments from Nannochloropsis salina and Nannochloropsis maritima using supercritical CO2 extraction: Effects of process parameters and pre-treatment. Journal of Supercritical Fluids, 2020, 165, pp.104934. ⟨10.1016/j.supflu.2020.104934⟩. ⟨hal-03232116⟩
Adil Mouahid, Kanitta Seengeon, Mathieu Martino, Christelle Crampon, Avery Kramer, et al.. Selective extraction of neutral lipids and pigments from Nannochloropsis salina and Nannochloropsis maritima using supercritical CO2 extraction: Effects of process parameters and pre-treatment. Journal of Supercritical Fluids, 2020, 165, pp.104934. ⟨10.1016/j.supflu.2020.104934⟩. ⟨hal-02960133⟩ Plus de détails...
Supercritical CO2 extraction experiments were conducted to investigate the effects of pretreatment and process parameters on neutral lipids, chlorophylls and carotenoids recovery on two species of Nannochloropsis. For Nannochloropsis maritima, a factorial experimental design was performed (P: [100-300] bar, T: [313-333] K). The highest extraction yields were obtained at the highest pressures and temperatures. Two drying modes, ring drying and air flow drying, were compared. Although total extraction yield and extraction kinetics were observed to be greater using air flow dried microalgae, extracts from this drying method resulted in partial degradation of glycerides in free fatty acids. Ring dried extracts maintained the same neutral lipid composition as the initial biomass. Based on these results, ring dried Nannochloropsis salina was extracted using supercritical CO2 at 333 K and both 300-400 bar. Extraction curves were modelled using the Sovova's mathematical model.
Adil Mouahid, Kanitta Seengeon, Mathieu Martino, Christelle Crampon, Avery Kramer, et al.. Selective extraction of neutral lipids and pigments from Nannochloropsis salina and Nannochloropsis maritima using supercritical CO2 extraction: Effects of process parameters and pre-treatment. Journal of Supercritical Fluids, 2020, 165, pp.104934. ⟨10.1016/j.supflu.2020.104934⟩. ⟨hal-02960133⟩
Cristian Barca, Mathieu Martino, Pierre Hennebert, Nicolas Roche. Kinetics and capacity of phosphorus extraction from solid residues obtained from wet air oxidation of sewage sludge. Waste Management, 2019, 89, pp.275-283. ⟨10.1016/j.wasman.2019.04.024⟩. ⟨hal-02118222⟩ Plus de détails...
Solid residues from thermal treatments of sewage sludge (SS) represent a valuable source of phosphorus (P) for the fertilizer production. This study aims at evaluating the P recovery potential from solid residues obtained from wet air oxidation of SS under subcritical water conditions (WAO residues). A series of P extraction experiments was performed by acidic and alkaline leaching at different liquid to solid ratios. Hot chemical extractions and P fractionations were also carried out to characterize the chemical composition of the WAO residues. The main objectives of this work were to determine the best operating conditions for P extraction, and to describe and understand the kinetics and the main mechanisms leading to P release. The results obtained in this study indicate that 1 M citric acid and 1 M HCl at the liquid to solid ratio of 10 L/kg can extract 61% and 65% of the total P content after 2 h of contact time at room temperature, thus giving P extraction capacities of 81 and 86 g P/kg WAO residues, respectively. The analysis of kinetic data indicates that P extraction with 1 M HCl is faster, but 1 M citric acid can give higher P extraction efficiencies at the equilibrium. The molar ratios of Ca to P of the leachates suggest that P extraction from WAO residues was primarily due to the dissolution of a mixture of various Ca-P complexes. (C) 2019 Elsevier Ltd. All rights reserved.
Cristian Barca, Mathieu Martino, Pierre Hennebert, Nicolas Roche. Kinetics and capacity of phosphorus extraction from solid residues obtained from wet air oxidation of sewage sludge. Waste Management, 2019, 89, pp.275-283. ⟨10.1016/j.wasman.2019.04.024⟩. ⟨hal-02118222⟩
Cristian Barca, Mathieu Martino, Pierre Hennebert, Nicolas Roche. Kinetics and capacity of phosphorus extraction from solid residues obtained from wet air oxidation of sewage sludge. Waste Management, Elsevier, 2019, 89, pp.275-283. ⟨10.1016/j.wasman.2019.04.024⟩. ⟨hal-02118222⟩ Plus de détails...
Solid residues from thermal treatments of sewage sludge (SS) represent a valuable source of phosphorus (P) for the fertilizer production. This study aims at evaluating the P recovery potential from solid residues obtained from wet air oxidation of SS under subcritical water conditions (WAO residues). A series of P extraction experiments was performed by acidic and alkaline leaching at different liquid to solid ratios. Hot chemical extractions and P fractionations were also carried out to characterize the chemical composition of the WAO residues. The main objectives of this work were to determine the best operating conditions for P extraction, and to describe and understand the kinetics and the main mechanisms leading to P release. The results obtained in this study indicate that 1 M citric acid and 1 M HCl at the liquid to solid ratio of 10 L/kg can extract 61% and 65% of the total P content after 2 h of contact time at room temperature, thus giving P extraction capacities of 81 and 86 g P/kg WAO residues, respectively. The analysis of kinetic data indicates that P extraction with 1 M HCl is faster, but 1 M citric acid can give higher P extraction efficiencies at the equilibrium. The molar ratios of Ca to P of the leachates suggest that P extraction from WAO residues was primarily due to the dissolution of a mixture of various Ca-P complexes. (C) 2019 Elsevier Ltd. All rights reserved.
Cristian Barca, Mathieu Martino, Pierre Hennebert, Nicolas Roche. Kinetics and capacity of phosphorus extraction from solid residues obtained from wet air oxidation of sewage sludge. Waste Management, Elsevier, 2019, 89, pp.275-283. ⟨10.1016/j.wasman.2019.04.024⟩. ⟨hal-02118222⟩
Eric Serre, Sandrine Hugues, Emilia Crespo del Arco, Anthony Randriamampianina, Patrick Bontoux. Axisymmetric and three-dimensional instabilities in an Ekman boundary layer flow. International Journal of Heat and Fluid Flow, 2001, 22 (1), pp.82-93. ⟨hal-01023080⟩ Plus de détails...
Eric Serre, Sandrine Hugues, Emilia Crespo del Arco, Anthony Randriamampianina, Patrick Bontoux. Axisymmetric and three-dimensional instabilities in an Ekman boundary layer flow. International Journal of Heat and Fluid Flow, 2001, 22 (1), pp.82-93. ⟨hal-01023080⟩
Journal: International Journal of Heat and Fluid Flow
